Summary
This strain contains 20 protein-level mutations:
- 6 mutations in Spike protein: L5F, HV 69-70 deletion, T95I, D614G, T859N, D950H
- 2 mutations in N protein: T205I, M234I
- 2 mutations in NS3 protein: P42L, Q57H
- 2 mutations in NS8 protein: T11I, A51S
- 1 mutation in NS9c protein: L52F
- 1 mutation in NSP2 protein: T85I
- 3 mutations in NSP4 protein: K399E, L438P, A446V
- 2 mutations in NSP6 protein: SGF 106-108 deletion, V278I
- 1 mutation in NSP12 protein: P323L
We identified all possible linear viral peptides affected by these mutations. Whenever it was possible, we matched the reference peptide with the mutated one. For example, D -> L mutation transformed SDNGPQNQR to SLNGPQNQR. Cases when it was not meaningful included deletions and insertions at the flanks of the peptide, e.g., HV deletion in NVTWFHAIHV peptide.
Then, we predicted binding affinities between the selected peptides and frequent HLA alleles. Predictions were made with NetMHCpan-4.1 and NetMHCIIpan-4.0. The binding affinities were classifies into three groups:
- Tight binding (IC50 affinity ≤ 50 nM)
- Moderate binding (50 nM < IC50 affinity ≤ 500 nM)
- Weak/no binding (IC50 affinity > 500 nM)
Here we report HLA-peptide interactions whose affinity was altered by at least two folds. Note that mutations with empty set of altered interactions are not showed.
The total number of interactions between HLA-DRB1*15:01 and peptides affected by the mutations
Weaker binding was found for 164 epitopes, while stronger binding was found for 191 entries. There are 916 tight binders for the HLA-DRB1*15:01 in the reference immunopeptidome (IC50 affinity ≤ 50 nM); 32 of them became non-tight binders (3.5%) and 0 novel tight binders appeared (0.0%). To avoid possible divisions by zero, we used + 1 regularization term in denominators when calculating percentages.
Spike protein
HV 69-70 deletion
Reference RSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFAST
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Mutated RSSVLHSTQDLFLPFFSNVTWFHAI‑‑SGTNGTKRFDNPVLPFNDGVYFASI
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | PFFSNVTWFHAIHVS | PFFSNVTWFHAIS | 19 | - |
| HLA-DRB1*15:01 | FFSNVTWFHAIHVSG | FFSNVTWFHAISG | 19 | - |
| HLA-DRB1*15:01 | FSNVTWFHAIHVSGT | FSNVTWFHAISGT | 19 | - |
| HLA-DRB1*15:01 | SNVTWFHAIHVSGTN | SNVTWFHAISGTN | 26 | - |
| HLA-DRB1*15:01 | LPFFSNVTWFHAIHV | LPFFSNVTWFHAI | 30 | - |
| HLA-DRB1*15:01 | FFSNVTWFHAIHVSGT | FFSNVTWFHAISGT | 35 | - |
| HLA-DRB1*15:01 | PFFSNVTWFHAIHVSG | PFFSNVTWFHAISG | 36 | - |
| HLA-DRB1*15:01 | LPFFSNVTWFHAIHVS | LPFFSNVTWFHAIS | 37 | - |
| HLA-DRB1*15:01 | FSNVTWFHAIHVSGTN | FSNVTWFHAISGTN | 37 | - |
| HLA-DRB1*15:01 | NVTWFHAIHVSGTNG | NVTWFHAISGTNG | 37 | - |
| HLA-DRB1*15:01 | TQDLFLPFFSNVTWFHAIHV | - | 43 | - |
| HLA-DRB1*15:01 | LFLPFFSNVTWFHAIHV | - | 50 | - |
| HLA-DRB1*15:01 | SNVTWFHAIHVSGTNG | SNVTWFHAISGTNG | 57 | - |
| HLA-DRB1*15:01 | FLPFFSNVTWFHAIHV | FLPFFSNVTWFHAI | 60 | - |
| HLA-DRB1*15:01 | QDLFLPFFSNVTWFHAIHV | - | 64 | - |
| HLA-DRB1*15:01 | STQDLFLPFFSNVTWFHAIH | - | 72 | - |
| HLA-DRB1*15:01 | STQDLFLPFFSNVTWFHAIHVS | STQDLFLPFFSNVTWFHAIS | - | 73 |
| HLA-DRB1*15:01 | TQDLFLPFFSNVTWFHAIHVSG | TQDLFLPFFSNVTWFHAISG | - | 76 |
| HLA-DRB1*15:01 | QDLFLPFFSNVTWFHAIHVSGT | QDLFLPFFSNVTWFHAISGT | - | 81 |
| HLA-DRB1*15:01 | NVTWFHAIHVSGTNGT | NVTWFHAISGTNGT | 81 | - |
| HLA-DRB1*15:01 | DLFLPFFSNVTWFHAIHVSGTN | DLFLPFFSNVTWFHAISGTN | - | 101 |
| HLA-DRB1*15:01 | DLFLPFFSNVTWFHAIH | - | 104 | - |
| HLA-DRB1*15:01 | TQDLFLPFFSNVTWFHAIH | - | 111 | - |
| HLA-DRB1*15:01 | TQDLFLPFFSNVTWFHAIHVS | TQDLFLPFFSNVTWFHAIS | - | 112 |
| HLA-DRB1*15:01 | LFLPFFSNVTWFHAIHVSGTNG | LFLPFFSNVTWFHAISGTNG | - | 117 |
| HLA-DRB1*15:01 | VTWFHAIHVSGTNGT | VTWFHAISGTNGT | 123 | - |
| HLA-DRB1*15:01 | QDLFLPFFSNVTWFHAIHVSG | QDLFLPFFSNVTWFHAISG | - | 124 |
| HLA-DRB1*15:01 | DLFLPFFSNVTWFHAIHV | - | 135 | - |
| HLA-DRB1*15:01 | FLPFFSNVTWFHAIHVSGTNGT | FLPFFSNVTWFHAISGTNGT | - | 137 |
| HLA-DRB1*15:01 | LPFFSNVTWFHAIHVSGTNGTK | LPFFSNVTWFHAISGTNGTK | - | 150 |
| HLA-DRB1*15:01 | FLPFFSNVTWFHAIH | FLPFFSNVTWFHAI | 155 | - |
| HLA-DRB1*15:01 | PFFSNVTWFHAIHVSGTNGTKR | PFFSNVTWFHAISGTNGTKR | - | 156 |
| HLA-DRB1*15:01 | DLFLPFFSNVTWFHAIHVSGT | DLFLPFFSNVTWFHAISGT | - | 178 |
| HLA-DRB1*15:01 | FFSNVTWFHAIHVSGTNGTKRF | FFSNVTWFHAISGTNGTKRF | - | 182 |
| HLA-DRB1*15:01 | LFLPFFSNVTWFHAIH | - | 191 | - |
| HLA-DRB1*15:01 | LFLPFFSNVTWFHAIHVSGTN | LFLPFFSNVTWFHAISGTN | - | 192 |
| HLA-DRB1*15:01 | FLPFFSNVTWFHAIHVSGTNG | FLPFFSNVTWFHAISGTNG | - | 217 |
| HLA-DRB1*15:01 | QDLFLPFFSNVTWFHAIH | - | 222 | - |
| HLA-DRB1*15:01 | FSNVTWFHAIHVSGTNGTKRFD | FSNVTWFHAISGTNGTKRFD | - | 223 |
| HLA-DRB1*15:01 | LPFFSNVTWFHAIHVSGTNGT | LPFFSNVTWFHAISGTNGT | - | 239 |
| HLA-DRB1*15:01 | PFFSNVTWFHAIHVSGTNGTK | PFFSNVTWFHAISGTNGTK | - | 261 |
| HLA-DRB1*15:01 | FFSNVTWFHAIHVSGTNGTKR | FFSNVTWFHAISGTNGTKR | - | 294 |
| HLA-DRB1*15:01 | VTWFHAIHVSGTNGTK | VTWFHAISGTNGTK | 296 | - |
| HLA-DRB1*15:01 | FSNVTWFHAIHVSGTNGTKRF | FSNVTWFHAISGTNGTKRF | - | 348 |
| HLA-DRB1*15:01 | SNVTWFHAIHVSGTNGTKRFDN | SNVTWFHAISGTNGTKRFDN | - | 364 |
| HLA-DRB1*15:01 | NVTWFHAIHVSGTNGTKRFD | NVTWFHAISGTNGTKRFD | 79 | 2301 |
| HLA-DRB1*15:01 | SNVTWFHAIHVSGTNGTKRF | SNVTWFHAISGTNGTKRF | 48 | 1300 |
| HLA-DRB1*15:01 | FSNVTWFHAIHVSGTNGTKR | FSNVTWFHAISGTNGTKR | 38 | 721 |
| HLA-DRB1*15:01 | FFSNVTWFHAIHVSGTNGTK | FFSNVTWFHAISGTNGTK | 35 | 589 |
| HLA-DRB1*15:01 | VTWFHAIHVSGTNGTKRFDN | VTWFHAISGTNGTKRFDN | 275 | 4303 |
| HLA-DRB1*15:01 | PFFSNVTWFHAIHVSGTNGT | PFFSNVTWFHAISGTNGT | 33 | 515 |
| HLA-DRB1*15:01 | LPFFSNVTWFHAIHVSGTNG | LPFFSNVTWFHAISGTNG | 32 | 468 |
| HLA-DRB1*15:01 | FLPFFSNVTWFHAIHVSGTN | FLPFFSNVTWFHAISGTN | 31 | 432 |
| HLA-DRB1*15:01 | LFLPFFSNVTWFHAIHVSGT | LFLPFFSNVTWFHAISGT | 31 | 413 |
| HLA-DRB1*15:01 | DLFLPFFSNVTWFHAIHVSG | DLFLPFFSNVTWFHAISG | 31 | 357 |
| HLA-DRB1*15:01 | QDLFLPFFSNVTWFHAIHVS | QDLFLPFFSNVTWFHAIS | 31 | 225 |
| HLA-DRB1*15:01 | FLPFFSNVTWFHAIHVS | FLPFFSNVTWFHAIS | 32 | 165 |
| HLA-DRB1*15:01 | NVTWFHAIHVSGTNGTKRF | NVTWFHAISGTNGTKRF | 121 | 578 |
| HLA-DRB1*15:01 | SNVTWFHAIHVSGTNGTKR | SNVTWFHAISGTNGTKR | 77 | 342 |
| HLA-DRB1*15:01 | LPFFSNVTWFHAIHVSG | LPFFSNVTWFHAISG | 32 | 139 |
| HLA-DRB1*15:01 | FLPFFSNVTWFHAIHVSGT | FLPFFSNVTWFHAISGT | 44 | 172 |
| HLA-DRB1*15:01 | FSNVTWFHAIHVSGTNGTK | FSNVTWFHAISGTNGTK | 53 | 192 |
| HLA-DRB1*15:01 | NVTWFHAIHVSGTNGTK | NVTWFHAISGTNGTK | 77 | 272 |
| HLA-DRB1*15:01 | LFLPFFSNVTWFHAIHVSG | LFLPFFSNVTWFHAISG | 47 | 157 |
| HLA-DRB1*15:01 | FFSNVTWFHAIHVSGTNGT | FFSNVTWFHAISGTNGT | 48 | 160 |
| HLA-DRB1*15:01 | LPFFSNVTWFHAIHVSGTN | LPFFSNVTWFHAISGTN | 45 | 142 |
| HLA-DRB1*15:01 | PFFSNVTWFHAIHVSGTNG | PFFSNVTWFHAISGTNG | 46 | 144 |
| HLA-DRB1*15:01 | PFFSNVTWFHAIHVSGT | PFFSNVTWFHAISGT | 31 | 88 |
| HLA-DRB1*15:01 | SNVTWFHAIHVSGTNGT | SNVTWFHAISGTNGT | 53 | 146 |
| HLA-DRB1*15:01 | FLPFFSNVTWFHAIHVSG | FLPFFSNVTWFHAISG | 102 | 278 |
| HLA-DRB1*15:01 | VTWFHAIHVSGTNGTKRFD | VTWFHAISGTNGTKRFD | 444 | 1187 |
| HLA-DRB1*15:01 | FSNVTWFHAIHVSGTNG | FSNVTWFHAISGTNG | 33 | 78 |
| HLA-DRB1*15:01 | FFSNVTWFHAIHVSGTN | FFSNVTWFHAISGTN | 31 | 69 |
| HLA-DRB1*15:01 | LPFFSNVTWFHAIHVSGT | LPFFSNVTWFHAISGT | 97 | 214 |
| HLA-DRB1*15:01 | DLFLPFFSNVTWFHAIHVS | DLFLPFFSNVTWFHAIS | 49 | 107 |
| HLA-DRB1*15:01 | NVTWFHAIHVSGTNGTKR | NVTWFHAISGTNGTKR | 308 | 642 |
| HLA-DRB1*15:01 | LFLPFFSNVTWFHAIHVS | LFLPFFSNVTWFHAIS | 96 | 199 |
T95I
Reference VSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIV
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Mutated ‑SGTNGTKRFDNPVLPFNDGVYFASIEKSNIIRGWIFGTTLDSKTQSLLIV
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | PFNDGVYFASTEKSNIIRGW | PFNDGVYFASIEKSNIIRGW | 972 | 389 |
| HLA-DRB1*15:01 | FNDGVYFASTEKSNIIRGWI | FNDGVYFASIEKSNIIRGWI | 915 | 370 |
| HLA-DRB1*15:01 | LPFNDGVYFASTEKSNIIRG | LPFNDGVYFASIEKSNIIRG | 937 | 402 |
| HLA-DRB1*15:01 | FNDGVYFASTEKSNI | FNDGVYFASIEKSNI | 1061 | 470 |
| HLA-DRB1*15:01 | NDGVYFASTEKSNIIRGWIF | NDGVYFASIEKSNIIRGWIF | 800 | 367 |
| HLA-DRB1*15:01 | DGVYFASTEKSNIIR | DGVYFASIEKSNIIR | 953 | 441 |
| HLA-DRB1*15:01 | DGVYFASTEKSNIIRGWIFG | DGVYFASIEKSNIIRGWIFG | 822 | 383 |
| HLA-DRB1*15:01 | VLPFNDGVYFASTEKSNIIR | VLPFNDGVYFASIEKSNIIR | 811 | 386 |
| HLA-DRB1*15:01 | FASTEKSNIIRGWIF | FASIEKSNIIRGWIF | 820 | 391 |
D950H
Reference NQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISS
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Mutated NQFNSAIGKIQDSLSSTASALGKLQHVVNQNAQALNTLVKQLSSNFGAISS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | ALGKLQDVVNQNAQA | ALGKLQHVVNQNAQA | 1111 | 165 |
| HLA-DRB1*15:01 | SALGKLQDVVNQNAQ | SALGKLQHVVNQNAQ | 1820 | 273 |
| HLA-DRB1*15:01 | ASALGKLQDVVNQNAQA | ASALGKLQHVVNQNAQA | 2370 | 390 |
| HLA-DRB1*15:01 | SALGKLQDVVNQNAQA | SALGKLQHVVNQNAQA | 2153 | 377 |
| HLA-DRB1*15:01 | SALGKLQDVVNQNAQAL | SALGKLQHVVNQNAQAL | 1450 | 260 |
| HLA-DRB1*15:01 | LGKLQDVVNQNAQAL | LGKLQHVVNQNAQAL | 678 | 124 |
| HLA-DRB1*15:01 | ALGKLQDVVNQNAQAL | ALGKLQHVVNQNAQAL | 1359 | 249 |
| HLA-DRB1*15:01 | ALGKLQDVVNQNAQALN | ALGKLQHVVNQNAQALN | 1265 | 249 |
| HLA-DRB1*15:01 | SSTASALGKLQDVVNQNAQA | SSTASALGKLQHVVNQNAQA | 1986 | 402 |
| HLA-DRB1*15:01 | TASALGKLQDVVNQNAQAL | TASALGKLQHVVNQNAQAL | 2042 | 423 |
| HLA-DRB1*15:01 | LGKLQDVVNQNAQALN | LGKLQHVVNQNAQALN | 1168 | 250 |
| HLA-DRB1*15:01 | LGKLQDVVNQNAQALNT | LGKLQHVVNQNAQALNT | 1184 | 262 |
| HLA-DRB1*15:01 | STASALGKLQDVVNQNAQAL | STASALGKLQHVVNQNAQAL | 1192 | 266 |
| HLA-DRB1*15:01 | ASALGKLQDVVNQNAQALN | ASALGKLQHVVNQNAQALN | 1720 | 384 |
| HLA-DRB1*15:01 | GKLQDVVNQNAQALN | GKLQHVVNQNAQALN | 703 | 166 |
| HLA-DRB1*15:01 | SALGKLQDVVNQNAQALNT | SALGKLQHVVNQNAQALNT | 1501 | 359 |
| HLA-DRB1*15:01 | TASALGKLQDVVNQNAQALN | TASALGKLQHVVNQNAQALN | 907 | 230 |
| HLA-DRB1*15:01 | GKLQDVVNQNAQALNT | GKLQHVVNQNAQALNT | 1266 | 348 |
| HLA-DRB1*15:01 | ASALGKLQDVVNQNAQALNT | ASALGKLQHVVNQNAQALNT | 764 | 212 |
| HLA-DRB1*15:01 | ALGKLQDVVNQNAQALNTL | ALGKLQHVVNQNAQALNTL | 1136 | 317 |
| HLA-DRB1*15:01 | LGKLQDVVNQNAQALNTLV | LGKLQHVVNQNAQALNTLV | 1164 | 345 |
| HLA-DRB1*15:01 | GKLQDVVNQNAQALNTL | GKLQHVVNQNAQALNTL | 1025 | 322 |
| HLA-DRB1*15:01 | SALGKLQDVVNQNAQALNTL | SALGKLQHVVNQNAQALNTL | 550 | 180 |
| HLA-DRB1*15:01 | ALGKLQDVVNQNAQALNTLV | ALGKLQHVVNQNAQALNTLV | 534 | 185 |
| HLA-DRB1*15:01 | KLQDVVNQNAQALNT | KLQHVVNQNAQALNT | 714 | 251 |
| HLA-DRB1*15:01 | GKLQDVVNQNAQALNTLVK | GKLQHVVNQNAQALNTLVK | 1318 | 478 |
| HLA-DRB1*15:01 | LGKLQDVVNQNAQALNTLVK | LGKLQHVVNQNAQALNTLVK | 526 | 199 |
| HLA-DRB1*15:01 | KLQDVVNQNAQALNTL | KLQHVVNQNAQALNTL | 1114 | 432 |
| HLA-DRB1*15:01 | KLQDVVNQNAQALNTLV | KLQHVVNQNAQALNTLV | 1191 | 493 |
| HLA-DRB1*15:01 | GKLQDVVNQNAQALNTLVKQ | GKLQHVVNQNAQALNTLVKQ | 589 | 256 |
NS3 protein
P42L
Reference QGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSASKIITLKK
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Mutated QGEIKDATPSDFVRATATIPIQASLLFGWLIVGVALLAVFHSASKIITLKK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | TIPIQASLPFGWLIVGVALL | TIPIQASLLFGWLIVGVALL | 456 | 1373 |
| HLA-DRB1*15:01 | TIPIQASLPFGWLIV | TIPIQASLLFGWLIV | 198 | 593 |
| HLA-DRB1*15:01 | ATIPIQASLPFGWLIVGVAL | ATIPIQASLLFGWLIVGVAL | 387 | 1056 |
| HLA-DRB1*15:01 | IPIQASLPFGWLIVG | IPIQASLLFGWLIVG | 269 | 726 |
| HLA-DRB1*15:01 | ATIPIQASLPFGWLIV | ATIPIQASLLFGWLIV | 489 | 1310 |
| HLA-DRB1*15:01 | TATIPIQASLPFGWLIVGVA | TATIPIQASLLFGWLIVGVA | 343 | 861 |
| HLA-DRB1*15:01 | ATATIPIQASLPFGWLIVGV | ATATIPIQASLLFGWLIVGV | 363 | 893 |
| HLA-DRB1*15:01 | RATATIPIQASLPFGWLIVG | RATATIPIQASLLFGWLIVG | 381 | 929 |
| HLA-DRB1*15:01 | ATIPIQASLPFGWLIVG | ATIPIQASLLFGWLIVG | 352 | 841 |
| HLA-DRB1*15:01 | TATIPIQASLPFGWLIV | TATIPIQASLLFGWLIV | 341 | 752 |
| HLA-DRB1*15:01 | TIPIQASLPFGWLIVGV | TIPIQASLLFGWLIVGV | 377 | 781 |
| HLA-DRB1*15:01 | VRATATIPIQASLPFGWLIV | VRATATIPIQASLLFGWLIV | 381 | 768 |
Q57H
Reference TATIPIQASLPFGWLIVGVALLAVFQSASKIITLKKRWQLALSKGVHFVCN
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Mutated TATIPIQASLLFGWLIVGVALLAVFHSASKIITLKKRWQLALSKGVHFVCN
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | LIVGVALLAVFQSAS | LIVGVALLAVFHSAS | 826 | 393 |
NS8 protein
T11I
Reference MKFLVFLGIITTVAAFHQECSLQSCTQHQPYVVDDP
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Mutated MKFLVFLGIIITVAAFHQECSLQSCTQHQPYVVDDP
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | FLVFLGIITTVAAFH | FLVFLGIIITVAAFH | 375 | 1133 |
NSP2 protein
T85I
Reference WYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEK
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Mutated WYTERSEKSYELQTPFEIKLAKKFDIFNGECPNFVFPLNSIIKTIQPRVEK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | EIKLAKKFDTFNGECPNFV | EIKLAKKFDIFNGECPNFV | 926 | 395 |
| HLA-DRB1*15:01 | PFEIKLAKKFDTFNG | PFEIKLAKKFDIFNG | 189 | 81 |
| HLA-DRB1*15:01 | TPFEIKLAKKFDTFNGEC | TPFEIKLAKKFDIFNGEC | 1154 | 498 |
| HLA-DRB1*15:01 | ELQTPFEIKLAKKFDTFN | ELQTPFEIKLAKKFDIFN | 985 | 431 |
| HLA-DRB1*15:01 | PFEIKLAKKFDTFNGECPN | PFEIKLAKKFDIFNGECPN | 459 | 201 |
| HLA-DRB1*15:01 | QTPFEIKLAKKFDTFNGE | QTPFEIKLAKKFDIFNGE | 1071 | 472 |
| HLA-DRB1*15:01 | LQTPFEIKLAKKFDTFNG | LQTPFEIKLAKKFDIFNG | 1072 | 474 |
| HLA-DRB1*15:01 | TPFEIKLAKKFDTFN | TPFEIKLAKKFDIFN | 162 | 72 |
| HLA-DRB1*15:01 | TPFEIKLAKKFDTFNGECP | TPFEIKLAKKFDIFNGECP | 421 | 188 |
| HLA-DRB1*15:01 | QTPFEIKLAKKFDTFNGEC | QTPFEIKLAKKFDIFNGEC | 398 | 180 |
| HLA-DRB1*15:01 | QTPFEIKLAKKFDTFNG | QTPFEIKLAKKFDIFNG | 285 | 129 |
| HLA-DRB1*15:01 | QTPFEIKLAKKFDTFN | QTPFEIKLAKKFDIFN | 307 | 139 |
| HLA-DRB1*15:01 | ELQTPFEIKLAKKFDTFNG | ELQTPFEIKLAKKFDIFNG | 392 | 178 |
| HLA-DRB1*15:01 | PFEIKLAKKFDTFNGEC | PFEIKLAKKFDIFNGEC | 317 | 144 |
| HLA-DRB1*15:01 | LQTPFEIKLAKKFDTFNGE | LQTPFEIKLAKKFDIFNGE | 382 | 175 |
| HLA-DRB1*15:01 | TPFEIKLAKKFDTFNGE | TPFEIKLAKKFDIFNGE | 289 | 133 |
| HLA-DRB1*15:01 | TPFEIKLAKKFDTFNG | TPFEIKLAKKFDIFNG | 339 | 157 |
| HLA-DRB1*15:01 | FEIKLAKKFDTFNGECPNF | FEIKLAKKFDIFNGECPNF | 517 | 240 |
| HLA-DRB1*15:01 | PFEIKLAKKFDTFNGE | PFEIKLAKKFDIFNGE | 357 | 167 |
| HLA-DRB1*15:01 | FEIKLAKKFDTFNGE | FEIKLAKKFDIFNGE | 192 | 90 |
| HLA-DRB1*15:01 | LQTPFEIKLAKKFDTFN | LQTPFEIKLAKKFDIFN | 255 | 120 |
| HLA-DRB1*15:01 | EIKLAKKFDTFNGECPN | EIKLAKKFDIFNGECPN | 616 | 292 |
| HLA-DRB1*15:01 | EIKLAKKFDTFNGEC | EIKLAKKFDIFNGEC | 354 | 168 |
| HLA-DRB1*15:01 | EIKLAKKFDTFNGECP | EIKLAKKFDIFNGECP | 725 | 346 |
| HLA-DRB1*15:01 | YELQTPFEIKLAKKFDTF | YELQTPFEIKLAKKFDIF | 1005 | 480 |
| HLA-DRB1*15:01 | QTPFEIKLAKKFDTF | QTPFEIKLAKKFDIF | 165 | 79 |
| HLA-DRB1*15:01 | FEIKLAKKFDTFNGEC | FEIKLAKKFDIFNGEC | 382 | 183 |
| HLA-DRB1*15:01 | EIKLAKKFDTFNGECPNFVF | EIKLAKKFDIFNGECPNFVF | 458 | 220 |
| HLA-DRB1*15:01 | FEIKLAKKFDTFNGECP | FEIKLAKKFDIFNGECP | 329 | 160 |
| HLA-DRB1*15:01 | LQTPFEIKLAKKFDTF | LQTPFEIKLAKKFDIF | 316 | 154 |
| HLA-DRB1*15:01 | LQTPFEIKLAKKFDT | LQTPFEIKLAKKFDI | 293 | 143 |
| HLA-DRB1*15:01 | PFEIKLAKKFDTFNGECPNF | PFEIKLAKKFDIFNGECPNF | 225 | 110 |
| HLA-DRB1*15:01 | ELQTPFEIKLAKKFDT | ELQTPFEIKLAKKFDI | 630 | 309 |
| HLA-DRB1*15:01 | YELQTPFEIKLAKKFDTFN | YELQTPFEIKLAKKFDIFN | 352 | 174 |
| HLA-DRB1*15:01 | FEIKLAKKFDTFNGECPNFV | FEIKLAKKFDIFNGECPNFV | 272 | 135 |
| HLA-DRB1*15:01 | LQTPFEIKLAKKFDTFNGEC | LQTPFEIKLAKKFDIFNGEC | 190 | 95 |
| HLA-DRB1*15:01 | TPFEIKLAKKFDTFNGECPN | TPFEIKLAKKFDIFNGECPN | 208 | 104 |
NSP4 protein
L438P
Reference EEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSY
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Mutated EEAALCTFLLNKEMYLKLRSDVLLPPTQYNRYLVLYNKYKYFSGAMDTTSY
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLA | SDVLLPPTQYNRYLV | 184 | 1077 |
| HLA-DRB1*15:01 | RSDVLLPLTQYNRYLA | RSDVLLPPTQYNRYLV | 385 | 2241 |
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLAL | SDVLLPPTQYNRYLVL | 246 | 1226 |
| HLA-DRB1*15:01 | RSDVLLPLTQYNRYL | RSDVLLPPTQYNRYL | 466 | 2294 |
| HLA-DRB1*15:01 | LRSDVLLPLTQYNRYLA | LRSDVLLPPTQYNRYLV | 351 | 1722 |
| HLA-DRB1*15:01 | DVLLPLTQYNRYLALY | DVLLPPTQYNRYLVLY | 203 | 946 |
| HLA-DRB1*15:01 | LKLRSDVLLPLTQYNRYLA | LKLRSDVLLPPTQYNRYLV | 399 | 1842 |
| HLA-DRB1*15:01 | DVLLPLTQYNRYLAL | DVLLPPTQYNRYLVL | 119 | 546 |
| HLA-DRB1*15:01 | RSDVLLPLTQYNRYLAL | RSDVLLPPTQYNRYLVL | 234 | 1060 |
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLALY | SDVLLPPTQYNRYLVLY | 190 | 838 |
| HLA-DRB1*15:01 | VLLPLTQYNRYLALY | VLLPPTQYNRYLVLY | 96 | 419 |
| HLA-DRB1*15:01 | KLRSDVLLPLTQYNRYLAL | KLRSDVLLPPTQYNRYLVL | 313 | 1354 |
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLALYN | SDVLLPPTQYNRYLVLYN | 445 | 1800 |
| HLA-DRB1*15:01 | VLLPLTQYNRYLALYN | VLLPPTQYNRYLVLYN | 157 | 633 |
| HLA-DRB1*15:01 | DVLLPLTQYNRYLALYN | DVLLPPTQYNRYLVLYN | 150 | 567 |
| HLA-DRB1*15:01 | LRSDVLLPLTQYNRYLALY | LRSDVLLPPTQYNRYLVLY | 273 | 1031 |
| HLA-DRB1*15:01 | LKLRSDVLLPLTQYNRYLAL | LKLRSDVLLPPTQYNRYLVL | 179 | 658 |
| HLA-DRB1*15:01 | KLRSDVLLPLTQYNRYLALY | KLRSDVLLPPTQYNRYLVLY | 162 | 585 |
| HLA-DRB1*15:01 | RSDVLLPLTQYNRYLALYN | RSDVLLPPTQYNRYLVLYN | 205 | 705 |
| HLA-DRB1*15:01 | LLPLTQYNRYLALYN | LLPPTQYNRYLVLYN | 80 | 273 |
| HLA-DRB1*15:01 | YLKLRSDVLLPLTQYNRYLA | YLKLRSDVLLPPTQYNRYLV | 188 | 628 |
| HLA-DRB1*15:01 | DVLLPLTQYNRYLALYNK | DVLLPPTQYNRYLVLYNK | 405 | 1340 |
| HLA-DRB1*15:01 | VLLPLTQYNRYLALYNK | VLLPPTQYNRYLVLYNK | 131 | 386 |
| HLA-DRB1*15:01 | LRSDVLLPLTQYNRYLALYN | LRSDVLLPPTQYNRYLVLYN | 123 | 353 |
| HLA-DRB1*15:01 | VLLPLTQYNRYLALYNKY | VLLPPTQYNRYLVLYNKY | 380 | 1086 |
| HLA-DRB1*15:01 | LLPLTQYNRYLALYNK | LLPPTQYNRYLVLYNK | 157 | 447 |
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLALYNK | SDVLLPPTQYNRYLVLYNK | 178 | 490 |
| HLA-DRB1*15:01 | LPLTQYNRYLALYNK | LPPTQYNRYLVLYNK | 79 | 208 |
| HLA-DRB1*15:01 | RSDVLLPLTQYNRYLALYNK | RSDVLLPPTQYNRYLVLYNK | 101 | 250 |
| HLA-DRB1*15:01 | LPLTQYNRYLALYNKY | LPPTQYNRYLVLYNKY | 165 | 406 |
| HLA-DRB1*15:01 | YLKLRSDVLLPLTQYNR | YLKLRSDVLLPPTQYNR | 443 | 1090 |
| HLA-DRB1*15:01 | DVLLPLTQYNRYLALYNKY | DVLLPPTQYNRYLVLYNKY | 165 | 401 |
| HLA-DRB1*15:01 | LLPLTQYNRYLALYNKY | LLPPTQYNRYLVLYNKY | 128 | 311 |
| HLA-DRB1*15:01 | LLPLTQYNRYLALYNKYK | LLPPTQYNRYLVLYNKYK | 349 | 838 |
| HLA-DRB1*15:01 | PLTQYNRYLALYNKY | PPTQYNRYLVLYNKY | 84 | 187 |
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLALYNKY | SDVLLPPTQYNRYLVLYNKY | 96 | 208 |
| HLA-DRB1*15:01 | LPLTQYNRYLALYNKYK | LPPTQYNRYLVLYNKYK | 117 | 250 |
| HLA-DRB1*15:01 | VLLPLTQYNRYLALYNKYK | VLLPPTQYNRYLVLYNKYK | 136 | 287 |
| HLA-DRB1*15:01 | YLKLRSDVLLPLTQY | YLKLRSDVLLPPTQY | 252 | 514 |
| HLA-DRB1*15:01 | KEMYLKLRSDVLLPLTQY | KEMYLKLRSDVLLPPTQY | 456 | 919 |
| HLA-DRB1*15:01 | PLTQYNRYLALYNKYK | PPTQYNRYLVLYNKYK | 161 | 323 |
A446V
Reference LLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHL
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated LLNKEMYLKLRSDVLLPPTQYNRYLVLYNKYKYFSGAMDTTSYREAACCHL
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLA | SDVLLPPTQYNRYLV | 184 | 1077 |
| HLA-DRB1*15:01 | RSDVLLPLTQYNRYLA | RSDVLLPPTQYNRYLV | 385 | 2241 |
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLAL | SDVLLPPTQYNRYLVL | 246 | 1226 |
| HLA-DRB1*15:01 | LRSDVLLPLTQYNRYLA | LRSDVLLPPTQYNRYLV | 351 | 1722 |
| HLA-DRB1*15:01 | DVLLPLTQYNRYLALY | DVLLPPTQYNRYLVLY | 203 | 946 |
| HLA-DRB1*15:01 | LKLRSDVLLPLTQYNRYLA | LKLRSDVLLPPTQYNRYLV | 399 | 1842 |
| HLA-DRB1*15:01 | DVLLPLTQYNRYLAL | DVLLPPTQYNRYLVL | 119 | 546 |
| HLA-DRB1*15:01 | RSDVLLPLTQYNRYLAL | RSDVLLPPTQYNRYLVL | 234 | 1060 |
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLALY | SDVLLPPTQYNRYLVLY | 190 | 838 |
| HLA-DRB1*15:01 | VLLPLTQYNRYLALY | VLLPPTQYNRYLVLY | 96 | 419 |
| HLA-DRB1*15:01 | KLRSDVLLPLTQYNRYLAL | KLRSDVLLPPTQYNRYLVL | 313 | 1354 |
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLALYN | SDVLLPPTQYNRYLVLYN | 445 | 1800 |
| HLA-DRB1*15:01 | VLLPLTQYNRYLALYN | VLLPPTQYNRYLVLYN | 157 | 633 |
| HLA-DRB1*15:01 | DVLLPLTQYNRYLALYN | DVLLPPTQYNRYLVLYN | 150 | 567 |
| HLA-DRB1*15:01 | LRSDVLLPLTQYNRYLALY | LRSDVLLPPTQYNRYLVLY | 273 | 1031 |
| HLA-DRB1*15:01 | LKLRSDVLLPLTQYNRYLAL | LKLRSDVLLPPTQYNRYLVL | 179 | 658 |
| HLA-DRB1*15:01 | KLRSDVLLPLTQYNRYLALY | KLRSDVLLPPTQYNRYLVLY | 162 | 585 |
| HLA-DRB1*15:01 | RSDVLLPLTQYNRYLALYN | RSDVLLPPTQYNRYLVLYN | 205 | 705 |
| HLA-DRB1*15:01 | LLPLTQYNRYLALYN | LLPPTQYNRYLVLYN | 80 | 273 |
| HLA-DRB1*15:01 | YLKLRSDVLLPLTQYNRYLA | YLKLRSDVLLPPTQYNRYLV | 188 | 628 |
| HLA-DRB1*15:01 | DVLLPLTQYNRYLALYNK | DVLLPPTQYNRYLVLYNK | 405 | 1340 |
| HLA-DRB1*15:01 | VLLPLTQYNRYLALYNK | VLLPPTQYNRYLVLYNK | 131 | 386 |
| HLA-DRB1*15:01 | LRSDVLLPLTQYNRYLALYN | LRSDVLLPPTQYNRYLVLYN | 123 | 353 |
| HLA-DRB1*15:01 | VLLPLTQYNRYLALYNKY | VLLPPTQYNRYLVLYNKY | 380 | 1086 |
| HLA-DRB1*15:01 | LLPLTQYNRYLALYNK | LLPPTQYNRYLVLYNK | 157 | 447 |
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLALYNK | SDVLLPPTQYNRYLVLYNK | 178 | 490 |
| HLA-DRB1*15:01 | LPLTQYNRYLALYNK | LPPTQYNRYLVLYNK | 79 | 208 |
| HLA-DRB1*15:01 | RSDVLLPLTQYNRYLALYNK | RSDVLLPPTQYNRYLVLYNK | 101 | 250 |
| HLA-DRB1*15:01 | LPLTQYNRYLALYNKY | LPPTQYNRYLVLYNKY | 165 | 406 |
| HLA-DRB1*15:01 | DVLLPLTQYNRYLALYNKY | DVLLPPTQYNRYLVLYNKY | 165 | 401 |
| HLA-DRB1*15:01 | LLPLTQYNRYLALYNKY | LLPPTQYNRYLVLYNKY | 128 | 311 |
| HLA-DRB1*15:01 | LLPLTQYNRYLALYNKYK | LLPPTQYNRYLVLYNKYK | 349 | 838 |
| HLA-DRB1*15:01 | PLTQYNRYLALYNKY | PPTQYNRYLVLYNKY | 84 | 187 |
| HLA-DRB1*15:01 | SDVLLPLTQYNRYLALYNKY | SDVLLPPTQYNRYLVLYNKY | 96 | 208 |
| HLA-DRB1*15:01 | LPLTQYNRYLALYNKYK | LPPTQYNRYLVLYNKYK | 117 | 250 |
| HLA-DRB1*15:01 | VLLPLTQYNRYLALYNKYK | VLLPPTQYNRYLVLYNKYK | 136 | 287 |
| HLA-DRB1*15:01 | PLTQYNRYLALYNKYK | PPTQYNRYLVLYNKYK | 161 | 323 |
NSP6 protein
SGF 106-108 deletion
Reference FNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYD
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated FNMVYMPASWVMRIMTWLDMVDTSL‑‑‑KLKDCVMYASAVVLLILMTARTVYD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | FKLKDCVMYASAVVL | KLKDCVMYASAVVL | 62 | - |
| HLA-DRB1*15:01 | GFKLKDCVMYASAVV | KLKDCVMYASAVV | 110 | - |
| HLA-DRB1*15:01 | GFKLKDCVMYASAVVLL | - | 117 | - |
| HLA-DRB1*15:01 | FKLKDCVMYASAVVLLI | - | 125 | - |
| HLA-DRB1*15:01 | SGFKLKDCVMYASAVVL | KLKDCVMYASAVVL | 126 | - |
| HLA-DRB1*15:01 | FKLKDCVMYASAVVLL | - | 163 | - |
| HLA-DRB1*15:01 | GFKLKDCVMYASAVVL | KLKDCVMYASAVVL | 171 | - |
| HLA-DRB1*15:01 | MVDTSLSGFKLKDCVMYASAVVL | MVDTSLKLKDCVMYASAVVL | - | 195 |
| HLA-DRB1*15:01 | VDTSLSGFKLKDCVMYASAVVLL | VDTSLKLKDCVMYASAVVLL | - | 204 |
| HLA-DRB1*15:01 | LSGFKLKDCVMYASAVV | LKLKDCVMYASAVV | 217 | - |
| HLA-DRB1*15:01 | DTSLSGFKLKDCVMYASAVVLLI | DTSLKLKDCVMYASAVVLLI | - | 224 |
| HLA-DRB1*15:01 | TSLSGFKLKDCVMYASAVVLLIL | TSLKLKDCVMYASAVVLLIL | - | 231 |
| HLA-DRB1*15:01 | SGFKLKDCVMYASAVVLLIL | - | 239 | - |
| HLA-DRB1*15:01 | SLSGFKLKDCVMYASAVVLLILM | SLKLKDCVMYASAVVLLILM | - | 252 |
| HLA-DRB1*15:01 | GFKLKDCVMYASAVVLLILM | - | 253 | - |
| HLA-DRB1*15:01 | DMVDTSLSGFKLKDCVMYASAVV | DMVDTSLKLKDCVMYASAVV | - | 264 |
| HLA-DRB1*15:01 | FKLKDCVMYASAVVLLILMT | - | 276 | - |
| HLA-DRB1*15:01 | LSGFKLKDCVMYASAVVLLILMT | LKLKDCVMYASAVVLLILMT | - | 282 |
| HLA-DRB1*15:01 | SGFKLKDCVMYASAVV | KLKDCVMYASAVV | 285 | - |
| HLA-DRB1*15:01 | VDTSLSGFKLKDCVMYASAVVL | VDTSLKLKDCVMYASAVVL | - | 295 |
| HLA-DRB1*15:01 | DTSLSGFKLKDCVMYASAVVLL | DTSLKLKDCVMYASAVVLL | - | 296 |
| HLA-DRB1*15:01 | SGFKLKDCVMYASAVVLLI | - | 314 | - |
| HLA-DRB1*15:01 | TSLSGFKLKDCVMYASAVVLLI | TSLKLKDCVMYASAVVLLI | - | 319 |
| HLA-DRB1*15:01 | GFKLKDCVMYASAVVLLIL | - | 333 | - |
| HLA-DRB1*15:01 | SLSGFKLKDCVMYASAVVLLIL | SLKLKDCVMYASAVVLLIL | - | 335 |
| HLA-DRB1*15:01 | FKLKDCVMYASAVVLLILM | - | 358 | - |
| HLA-DRB1*15:01 | LSGFKLKDCVMYASAVVLLILM | LKLKDCVMYASAVVLLILM | - | 361 |
| HLA-DRB1*15:01 | MRIMTWLDMVDTSLS | MRIMTWLDMVDTSL | 382 | - |
| HLA-DRB1*15:01 | SWVMRIMTWLDMVDTSLSGFKLK | SWVMRIMTWLDMVDTSLKLK | - | 401 |
| HLA-DRB1*15:01 | MVDTSLSGFKLKDCVMYASAVV | MVDTSLKLKDCVMYASAVV | - | 441 |
| HLA-DRB1*15:01 | ASWVMRIMTWLDMVDTSLSGFKL | ASWVMRIMTWLDMVDTSLKL | - | 459 |
| HLA-DRB1*15:01 | WVMRIMTWLDMVDTSLSGFKLKD | WVMRIMTWLDMVDTSLKLKD | - | 466 |
| HLA-DRB1*15:01 | LSGFKLKDCVMYASAVVL | LKLKDCVMYASAVVL | 630 | 61 |
| HLA-DRB1*15:01 | SLSGFKLKDCVMYASAVV | SLKLKDCVMYASAVV | 1025 | 108 |
| HLA-DRB1*15:01 | MRIMTWLDMVDTSLSGFK | MRIMTWLDMVDTSLK | 2429 | 340 |
NSP12 protein
P323L
Reference CVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHF
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated CVNCLDDRCILHCANFNVLFSTVFPLTSFGPLVRKIFVDGVPFVVSTGYHF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:01 | FSTVFPPTSFGPLVRK | FSTVFPLTSFGPLVRK | 2472 | 145 |
| HLA-DRB1*15:01 | STVFPPTSFGPLVRK | STVFPLTSFGPLVRK | 1187 | 73 |
| HLA-DRB1*15:01 | FSTVFPPTSFGPLVR | FSTVFPLTSFGPLVR | 1497 | 93 |
| HLA-DRB1*15:01 | STVFPPTSFGPLVRKI | STVFPLTSFGPLVRKI | 1911 | 131 |
| HLA-DRB1*15:01 | FSTVFPPTSFGPLVRKI | FSTVFPLTSFGPLVRKI | 1398 | 104 |
| HLA-DRB1*15:01 | TVFPPTSFGPLVRKI | TVFPLTSFGPLVRKI | 819 | 65 |
| HLA-DRB1*15:01 | LFSTVFPPTSFGPLVRKI | LFSTVFPLTSFGPLVRKI | 4430 | 355 |
| HLA-DRB1*15:01 | FSTVFPPTSFGPLVRKIF | FSTVFPLTSFGPLVRKIF | 3238 | 268 |
| HLA-DRB1*15:01 | LFSTVFPPTSFGPLVRK | LFSTVFPLTSFGPLVRK | 1458 | 125 |
| HLA-DRB1*15:01 | LFSTVFPPTSFGPLVR | LFSTVFPLTSFGPLVR | 2515 | 220 |
| HLA-DRB1*15:01 | TVFPPTSFGPLVRKIF | TVFPLTSFGPLVRKIF | 1138 | 110 |
| HLA-DRB1*15:01 | TVFPPTSFGPLVRKIFVD | TVFPLTSFGPLVRKIFVD | 3046 | 300 |
| HLA-DRB1*15:01 | STVFPPTSFGPLVRKIFV | STVFPLTSFGPLVRKIFV | 2782 | 277 |
| HLA-DRB1*15:01 | STVFPPTSFGPLVRKIF | STVFPLTSFGPLVRKIF | 895 | 92 |
| HLA-DRB1*15:01 | VFPPTSFGPLVRKIFVDG | VFPLTSFGPLVRKIFVDG | 3100 | 329 |
| HLA-DRB1*15:01 | VLFSTVFPPTSFGPLVRK | VLFSTVFPLTSFGPLVRK | 4258 | 469 |
| HLA-DRB1*15:01 | VFPPTSFGPLVRKIFV | VFPLTSFGPLVRKIFV | 1011 | 116 |
| HLA-DRB1*15:01 | LFSTVFPPTSFGPLVRKIF | LFSTVFPLTSFGPLVRKIF | 1264 | 147 |
| HLA-DRB1*15:01 | VFPPTSFGPLVRKIF | VFPLTSFGPLVRKIF | 482 | 57 |
| HLA-DRB1*15:01 | FPPTSFGPLVRKIFVD | FPLTSFGPLVRKIFVD | 1393 | 165 |
| HLA-DRB1*15:01 | VLFSTVFPPTSFGPLVRKI | VLFSTVFPLTSFGPLVRKI | 1477 | 177 |
| HLA-DRB1*15:01 | TVFPPTSFGPLVRKIFV | TVFPLTSFGPLVRKIFV | 774 | 96 |
| HLA-DRB1*15:01 | VFPPTSFGPLVRKIFVD | VFPLTSFGPLVRKIFVD | 857 | 108 |
| HLA-DRB1*15:01 | STVFPPTSFGPLVRKIFVD | STVFPLTSFGPLVRKIFVD | 1234 | 160 |
| HLA-DRB1*15:01 | FSTVFPPTSFGPLVRKIFV | FSTVFPLTSFGPLVRKIFV | 1114 | 146 |
| HLA-DRB1*15:01 | FPPTSFGPLVRKIFV | FPLTSFGPLVRKIFV | 551 | 76 |
| HLA-DRB1*15:01 | NVLFSTVFPPTSFGPLVRK | NVLFSTVFPLTSFGPLVRK | 1483 | 205 |
| HLA-DRB1*15:01 | FSTVFPPTSFGPLVRKIFVD | FSTVFPLTSFGPLVRKIFVD | 670 | 94 |
| HLA-DRB1*15:01 | TVFPPTSFGPLVRKIFVDG | TVFPLTSFGPLVRKIFVDG | 1261 | 177 |
| HLA-DRB1*15:01 | VLFSTVFPPTSFGPLVR | VLFSTVFPLTSFGPLVR | 1281 | 180 |
| HLA-DRB1*15:01 | FPPTSFGPLVRKIFVDG | FPLTSFGPLVRKIFVDG | 1102 | 155 |
| HLA-DRB1*15:01 | STVFPPTSFGPLVRKIFVDG | STVFPLTSFGPLVRKIFVDG | 705 | 100 |
| HLA-DRB1*15:01 | NVLFSTVFPPTSFGPLVRKI | NVLFSTVFPLTSFGPLVRKI | 700 | 102 |
| HLA-DRB1*15:01 | FPPTSFGPLVRKIFVDGV | FPLTSFGPLVRKIFVDGV | 3306 | 483 |
| HLA-DRB1*15:01 | VLFSTVFPPTSFGPLVRKIF | VLFSTVFPLTSFGPLVRKIF | 595 | 89 |
| HLA-DRB1*15:01 | LFSTVFPPTSFGPLVRKIFV | LFSTVFPLTSFGPLVRKIFV | 593 | 91 |
| HLA-DRB1*15:01 | PPTSFGPLVRKIFVDG | PLTSFGPLVRKIFVDG | 1963 | 311 |
| HLA-DRB1*15:01 | PPTSFGPLVRKIFVD | PLTSFGPLVRKIFVD | 902 | 143 |
| HLA-DRB1*15:01 | VFPPTSFGPLVRKIFVDGV | VFPLTSFGPLVRKIFVDGV | 1195 | 195 |
| HLA-DRB1*15:01 | TVFPPTSFGPLVRKIFVDGV | TVFPLTSFGPLVRKIFVDGV | 654 | 110 |
| HLA-DRB1*15:01 | VFPPTSFGPLVRKIFVDGVP | VFPLTSFGPLVRKIFVDGVP | 656 | 125 |
| HLA-DRB1*15:01 | LFSTVFPPTSFGPLV | LFSTVFPLTSFGPLV | 1566 | 306 |
| HLA-DRB1*15:01 | FNVLFSTVFPPTSFGPLVRK | FNVLFSTVFPLTSFGPLVRK | 615 | 122 |
| HLA-DRB1*15:01 | FPPTSFGPLVRKIFVDGVP | FPLTSFGPLVRKIFVDGVP | 1393 | 282 |
| HLA-DRB1*15:01 | PPTSFGPLVRKIFVDGV | PLTSFGPLVRKIFVDGV | 1259 | 277 |
| HLA-DRB1*15:01 | FNVLFSTVFPPTSFGPLVR | FNVLFSTVFPLTSFGPLVR | 1227 | 303 |
| HLA-DRB1*15:01 | ANFNVLFSTVFPPTS | ANFNVLFSTVFPLTS | 287 | 72 |
| HLA-DRB1*15:01 | HCANFNVLFSTVFPPTS | HCANFNVLFSTVFPLTS | 664 | 176 |
| HLA-DRB1*15:01 | NVLFSTVFPPTSFGPLV | NVLFSTVFPLTSFGPLV | 1293 | 348 |
| HLA-DRB1*15:01 | CANFNVLFSTVFPPTS | CANFNVLFSTVFPLTS | 873 | 236 |
| HLA-DRB1*15:01 | CANFNVLFSTVFPPT | CANFNVLFSTVFPLT | 400 | 110 |
| HLA-DRB1*15:01 | NFNVLFSTVFPPTSF | NFNVLFSTVFPLTSF | 272 | 78 |
| HLA-DRB1*15:01 | NVLFSTVFPPTSFGP | NVLFSTVFPLTSFGP | 674 | 198 |
| HLA-DRB1*15:01 | NFNVLFSTVFPPTSFGPLVR | NFNVLFSTVFPLTSFGPLVR | 571 | 168 |
| HLA-DRB1*15:01 | NVLFSTVFPPTSFGPL | NVLFSTVFPLTSFGPL | 1664 | 493 |
| HLA-DRB1*15:01 | FPPTSFGPLVRKIFVDGVPF | FPLTSFGPLVRKIFVDGVPF | 591 | 177 |
| HLA-DRB1*15:01 | HCANFNVLFSTVFPP | HCANFNVLFSTVFPL | 654 | 209 |
| HLA-DRB1*15:01 | NFNVLFSTVFPPTSFGP | NFNVLFSTVFPLTSFGP | 610 | 195 |
| HLA-DRB1*15:01 | LHCANFNVLFSTVFPPT | LHCANFNVLFSTVFPLT | 801 | 261 |
| HLA-DRB1*15:01 | NFNVLFSTVFPPTSFG | NFNVLFSTVFPLTSFG | 778 | 256 |
| HLA-DRB1*15:01 | CANFNVLFSTVFPPTSF | CANFNVLFSTVFPLTSF | 516 | 170 |
| HLA-DRB1*15:01 | PTSFGPLVRKIFVDG | LTSFGPLVRKIFVDG | 1116 | 369 |
| HLA-DRB1*15:01 | HCANFNVLFSTVFPPT | HCANFNVLFSTVFPLT | 1018 | 341 |
| HLA-DRB1*15:01 | ANFNVLFSTVFPPTSFG | ANFNVLFSTVFPLTSFG | 519 | 177 |
| HLA-DRB1*15:01 | ANFNVLFSTVFPPTSF | ANFNVLFSTVFPLTSF | 645 | 228 |
| HLA-DRB1*15:01 | FNVLFSTVFPPTSFG | FNVLFSTVFPLTSFG | 381 | 139 |
| HLA-DRB1*15:01 | VLFSTVFPPTSFGPL | VLFSTVFPLTSFGPL | 860 | 324 |
| HLA-DRB1*15:01 | FNVLFSTVFPPTSFGPL | FNVLFSTVFPLTSFGPL | 784 | 296 |
| HLA-DRB1*15:01 | PPTSFGPLVRKIFVDGVPF | PLTSFGPLVRKIFVDGVPF | 1177 | 454 |
| HLA-DRB1*15:01 | FNVLFSTVFPPTSFGP | FNVLFSTVFPLTSFGP | 1032 | 415 |
| HLA-DRB1*15:01 | NFNVLFSTVFPPTSFGPLV | NFNVLFSTVFPLTSFGPLV | 1075 | 460 |
| HLA-DRB1*15:01 | ILHCANFNVLFSTVFPP | ILHCANFNVLFSTVFPL | 866 | 387 |
| HLA-DRB1*15:01 | ILHCANFNVLFSTVFPPTS | ILHCANFNVLFSTVFPLTS | 912 | 424 |
| HLA-DRB1*15:01 | ANFNVLFSTVFPPTSFGPL | ANFNVLFSTVFPLTSFGPL | 955 | 453 |
| HLA-DRB1*15:01 | CANFNVLFSTVFPPTSFGP | CANFNVLFSTVFPLTSFGP | 950 | 474 |